|
|
| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
|
Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
30/06/2021 |
Actualizado : |
30/06/2021 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
CASTRO JANER, E.; DÍAZ, A.; FONTES, F.; BARAIBAR, F.; SAPORITI, T.; OLHAGARAY, M. E. |
Afiliación : |
ELEONOR CASTRO JANER, Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Montevideo, Uruguay.; ANDRÉ DÍAZ, Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Montevideo, Uruguay.; FLORENCIA FONTES, Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Montevideo, Uruguay.; FLORENCIA BARAIBAR, Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Montevideo, Uruguay.; TATIANA SAPORITI NOGUEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARÍA ERNESTINA OLHAGARAY, Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Montevideo, Uruguay. |
Título : |
Molecular survey of pyrethroid and fipronil resistance in isolates of Rhipicephalus microplus in the north of Uruguay. |
Fecha de publicación : |
2021 |
Fuente / Imprenta : |
Ticks and Tick-borne Diseases, 2021, Volume 12, Issue 5, Article number 101747. Doi: https://doi.org/10.1016/j.ttbdis.2021.101747 |
ISSN : |
1877-959X |
DOI : |
10.1016/j.ttbdis.2021.101747 |
Idioma : |
Inglés |
Notas : |
Article history: Received 23 July 2020; Received in revised form 29 March 2021; Accepted 6 April 2021; Available online 19 May 2021.
The authors are grateful to the funding of the Instituto Nacional de Investigación Agropecuaria-Plataforma de Salud Animal-Uruguay (CL_35).
Corresponding author: Janer, E.C.; Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Av. Lasplaces 1620, Montevideo, CP, Uruguay; email:elinorcastro59@gmail.com |
Contenido : |
ABSTRACT - The resistance of Rhipicephalus microplus to acaricides is a serious control problem, so its early diagnosis by a molecular technique is important. This study aims to develop a multiplex allele?specific polymerase chain reaction (PCR) for single-nucleotide polymorphisms (SNPs) in the para-sodium channel gene and in the GABA-Cl gene, associated with pyrethroids (cypermethrin and flumethrin) and fipronil resistance, respectively. We used 22 tick field isolates from farms with tick control problems (sampling convenience). These farms are located in departments of northern Uruguay. Three mutations in the sodium channel gene (Domain II S4?5: C190A and G215T; domain III S6: T2134A) and one in the GABA-Cl gene (A286S/L: CG856CC/TG) were studied. Mutations G215T and T213A were not detected. In all field isolates, the resistant allele (R) for C190A mutation (knockdown resistance, kdr) was detected, mainly in heterozygous individuals (SR) (11.1% to 86.7%). The highest incidence of the kdr mutant allele occurred in the Tacuarembó tick field isolates, where on 7 out of 10 farms >30% of individuals were SR and on one farm > 30% of individuals were RR. The next highest was Artigas (half of farms had>30% SR individuals and a quarter had >30% RR individuals). The resistance to dieldrin locus (rdl) mutation (CG856CC/TG) was absent in five field isolates. The highest incidenceof the mutant allele was observed in ticks from farms in Rivera (all farms had SR in >30% of individuals and two farms had RR in >12.5 and >16.7% of individuals) followed by farms in Tacuarembó (3 of 10 farms had >30% SR and 2 with >30% RR). Less than half of the farms had rdl in homozygous individuals. No significant association was observed between phenotypic bioassays and the rdl resistance allele. Several field isolates were phenotypically susceptible to the presence of the rdl allele. Several causes are possible (bioassay sensitivity, discriminating concentration). Individuals with simultaneous kdr and rdl mutations were present in 17 field isolates, and their frequency varied between 0.06% and 60%. Genotypic analysis shows that tick resistance to both acaricides, especially pyrethroids, is a serious problem. It is important to monitor the resistance using molecular techniques to plan efficient control measures. This is the first report describing kdr and rdl detection in R. microplus in Uruguay. © 2021 MenosABSTRACT - The resistance of Rhipicephalus microplus to acaricides is a serious control problem, so its early diagnosis by a molecular technique is important. This study aims to develop a multiplex allele?specific polymerase chain reaction (PCR) for single-nucleotide polymorphisms (SNPs) in the para-sodium channel gene and in the GABA-Cl gene, associated with pyrethroids (cypermethrin and flumethrin) and fipronil resistance, respectively. We used 22 tick field isolates from farms with tick control problems (sampling convenience). These farms are located in departments of northern Uruguay. Three mutations in the sodium channel gene (Domain II S4?5: C190A and G215T; domain III S6: T2134A) and one in the GABA-Cl gene (A286S/L: CG856CC/TG) were studied. Mutations G215T and T213A were not detected. In all field isolates, the resistant allele (R) for C190A mutation (knockdown resistance, kdr) was detected, mainly in heterozygous individuals (SR) (11.1% to 86.7%). The highest incidence of the kdr mutant allele occurred in the Tacuarembó tick field isolates, where on 7 out of 10 farms >30% of individuals were SR and on one farm > 30% of individuals were RR. The next highest was Artigas (half of farms had>30% SR individuals and a quarter had >30% RR individuals). The resistance to dieldrin locus (rdl) mutation (CG856CC/TG) was absent in five field isolates. The highest incidenceof the mutant allele was observed in ticks from farms in Rivera (all farms had SR in >30% of individuals an... Presentar Todo |
Palabras claves : |
Acaricides; Ixodidae; Reaxys retrieves substances; Rhipicephalus microplus. |
Asunto categoría : |
L73 Enfermedades de los animales |
Marc : |
LEADER 03773naa a2200265 a 4500 001 1062207 005 2021-06-30 008 2021 bl uuuu u00u1 u #d 022 $a1877-959X 024 7 $a10.1016/j.ttbdis.2021.101747$2DOI 100 1 $aCASTRO JANER, E. 245 $aMolecular survey of pyrethroid and fipronil resistance in isolates of Rhipicephalus microplus in the north of Uruguay.$h[electronic resource] 260 $c2021 500 $aArticle history: Received 23 July 2020; Received in revised form 29 March 2021; Accepted 6 April 2021; Available online 19 May 2021. The authors are grateful to the funding of the Instituto Nacional de Investigación Agropecuaria-Plataforma de Salud Animal-Uruguay (CL_35). Corresponding author: Janer, E.C.; Departamento de Patobiología, Unidad de Enfermedades Parasitarias, Facultad de Veterinaria, UDELAR. Av. Lasplaces 1620, Montevideo, CP, Uruguay; email:elinorcastro59@gmail.com 520 $aABSTRACT - The resistance of Rhipicephalus microplus to acaricides is a serious control problem, so its early diagnosis by a molecular technique is important. This study aims to develop a multiplex allele?specific polymerase chain reaction (PCR) for single-nucleotide polymorphisms (SNPs) in the para-sodium channel gene and in the GABA-Cl gene, associated with pyrethroids (cypermethrin and flumethrin) and fipronil resistance, respectively. We used 22 tick field isolates from farms with tick control problems (sampling convenience). These farms are located in departments of northern Uruguay. Three mutations in the sodium channel gene (Domain II S4?5: C190A and G215T; domain III S6: T2134A) and one in the GABA-Cl gene (A286S/L: CG856CC/TG) were studied. Mutations G215T and T213A were not detected. In all field isolates, the resistant allele (R) for C190A mutation (knockdown resistance, kdr) was detected, mainly in heterozygous individuals (SR) (11.1% to 86.7%). The highest incidence of the kdr mutant allele occurred in the Tacuarembó tick field isolates, where on 7 out of 10 farms >30% of individuals were SR and on one farm > 30% of individuals were RR. The next highest was Artigas (half of farms had>30% SR individuals and a quarter had >30% RR individuals). The resistance to dieldrin locus (rdl) mutation (CG856CC/TG) was absent in five field isolates. The highest incidenceof the mutant allele was observed in ticks from farms in Rivera (all farms had SR in >30% of individuals and two farms had RR in >12.5 and >16.7% of individuals) followed by farms in Tacuarembó (3 of 10 farms had >30% SR and 2 with >30% RR). Less than half of the farms had rdl in homozygous individuals. No significant association was observed between phenotypic bioassays and the rdl resistance allele. Several field isolates were phenotypically susceptible to the presence of the rdl allele. Several causes are possible (bioassay sensitivity, discriminating concentration). Individuals with simultaneous kdr and rdl mutations were present in 17 field isolates, and their frequency varied between 0.06% and 60%. Genotypic analysis shows that tick resistance to both acaricides, especially pyrethroids, is a serious problem. It is important to monitor the resistance using molecular techniques to plan efficient control measures. This is the first report describing kdr and rdl detection in R. microplus in Uruguay. © 2021 653 $aAcaricides 653 $aIxodidae 653 $aReaxys retrieves substances 653 $aRhipicephalus microplus 700 1 $aDÍAZ, A. 700 1 $aFONTES, F. 700 1 $aBARAIBAR, F. 700 1 $aSAPORITI, T. 700 1 $aOLHAGARAY, M. E. 773 $tTicks and Tick-borne Diseases, 2021, Volume 12, Issue 5, Article number 101747. Doi: https://doi.org/10.1016/j.ttbdis.2021.101747
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA Las Brujas (LB) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
Registro completo
|
Biblioteca (s) : |
INIA La Estanzuela. |
Fecha actual : |
21/02/2014 |
Actualizado : |
05/11/2018 |
Tipo de producción científica : |
Abstracts/Resúmenes |
Autor : |
ÁVILA, S.; CASALES, L.; BLANCO, P.H.; PÉREZ DE VIDA, F. |
Afiliación : |
MARIFLOR STELLA ÁVILA SILVA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; LUIS ALBERTO CASALES SOSA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; PEDRO HORACIO BLANCO BARRAL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; FERNANDO BLAS PEREZ DE VIDA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Resistencia varietal a mancha agregada de las vainas del arroz, rhizoctonia oryzae sativae, en Uruguay. [Cultivar resistance to aggregate sheath spot of rice, rhizoctonia oryze sativae, in Uruguay]. |
Fecha de publicación : |
1997 |
Fuente / Imprenta : |
ln: Congreso Latinoamericano de Fitopatologia, 9 : 1997 oct 12-17 : Montevideo Libro de resumenes. Montevideo (Uruguay): Sociedad Uruguaya de Fitopatologia, 1997. |
Páginas : |
p.66 |
Idioma : |
Español |
Thesagro : |
ARROZ; ENFERMEDADES DE LAS PLANTAS; MANCHAS; RESISTENCIA A LA ENFERMEDAD; RHIZOCTONIA; URUGUAY; VARIEDADES. |
Asunto categoría : |
-- |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/11800/1/congreso-latinoamericano-de-fitopatologia-9.-Libro-resumenes-1997-p.66.pdf
|
Marc : |
LEADER 00890naa a2200241 a 4500 001 1045352 005 2018-11-05 008 1997 bl uuuu u00u1 u #d 100 1 $aÁVILA, S. 245 $aResistencia varietal a mancha agregada de las vainas del arroz, rhizoctonia oryzae sativae, en Uruguay. [Cultivar resistance to aggregate sheath spot of rice, rhizoctonia oryze sativae, in Uruguay]. 260 $c1997 300 $ap.66 650 $aARROZ 650 $aENFERMEDADES DE LAS PLANTAS 650 $aMANCHAS 650 $aRESISTENCIA A LA ENFERMEDAD 650 $aRHIZOCTONIA 650 $aURUGUAY 650 $aVARIEDADES 700 1 $aCASALES, L. 700 1 $aBLANCO, P.H. 700 1 $aPÉREZ DE VIDA, F. 773 $tln: Congreso Latinoamericano de Fitopatologia, 9 : 1997 oct 12-17 : Montevideo Libro de resumenes. Montevideo (Uruguay): Sociedad Uruguaya de Fitopatologia, 1997.
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA La Estanzuela (LE) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
Expresión de búsqueda válido. Check! |
|
|